Synthetic controls with fungal ITS2

Here we consider some environmental fungi, mock communities, and a synthetic spike-in control using some of the Illumina data from the following paper:

Palmer et al. (2018) Non-biological synthetic spike-in controls and the AMPtk software pipeline improve mycobiome data. https://doi.org/10.7717/peerj.4925 https://www.ebi.ac.uk/ena/data/view/PRJNA305924

The mock communities have known composition, while the synthetic control has a mix of 12 artificial sequences which can be easily distinguished from the biological ITS2 sequences.

• Marker data
• Minimum Abundance Threshold