Soil Nematode Mock Community

Here we consider a mock community of over 20 soil nematodes (in triplicate), sequeced separately with four markers, with no-template PCR control blanks:

Ahmed et al. (2019) Metabarcoding of soil nematodes: the importance of taxonomic coverage and availability of reference sequences in choosing suitable marker(s) https://doi.org/10.3897/mbmg.3.36408 https://www.ebi.ac.uk/ena/data/view/PRJEB27581

This example requires creating a database covering the four different marker primers and known sequences (all of which ought to be properly curated).

• Marker data
• High level overview
• Presence and absence