Soil Nematode Mock Community

Here we consider a mock community of over 20 soil nematodes (in triplicate), sequeced separately with four markers, with no-template PCR control blanks:

Ahmed et al. (2019) Metabarcoding of soil nematodes: the importance of taxonomic coverage and availability of reference sequences in choosing suitable marker(s)

This example requires creating a database covering the four different marker primers and known sequences (all of which ought to be properly curated).