Pest Insect Mock Communities

Here we consider mock communities of up to six insect species, sequenced with three pooled markers (18S, 12S, COI), with no-template PCR control blanks:

Batovska et al. (2021) Developing a non-destructive metabarcoding protocol for detection of pest insects in bulk trap catches

This example requires creating a database covering the three different marker primers and known sequences (all of which ought to be properly curated).