Pest Insect Mock Communities
Here we consider mock communities of up to six insect species, sequenced with three pooled markers (18S, 12S, COI), with no-template PCR control blanks:
Batovska et al. (2021) Developing a non-destructive metabarcoding protocol for detection of pest insects in bulk trap catches https://doi.org/10.1038/s41598-021-85855-6 https://www.ebi.ac.uk/ena/data/view/PRJNA716058 https://zenodo.org/record/3557020 https://github.com/alexpiper/HemipteraMetabarcodingMS
This example requires creating a database covering the three different marker primers and known sequences (all of which ought to be properly curated).