Fungal Mock Community ITS1 & 2
Here we consider mock communities of 19 fungal sequences (in both equal and staggered ratios), prepared with various protocols, and negative controls.
This example is based on the two amplicon sequencing libraries from this paper:
Bakker (2018) A fungal mock community control for amplicon sequencing experiments. https://doi.org/10.1111/1755-0998.12760
The first library used a single primer set targeting ITS1, while the second library used two sets of primers targeting a different region of ITS1, and ITS2.