Endangered Species Mixes 16S etc
This is the most complicated of the examples considered, where most of the samples are “Experimental mixtures” of multiple plants and animals (plus two traditional medicine mixtures where the exact content is unknown), which have all been sequenced with about a dozen different primer pairs for multiple metabarcoding markers including 16S, COI, cyt-b, matK, rbcL, trnL and ITS2:
Arulandhu et al. (2017) Development and validation of a multi-locus DNA metabarcoding method to identify endangered species in complex samples. https://doi.org/10.1093/gigascience/gix080
This example requires creating a database of multiple markers (all of which ought to be properly curated). Both per-marker reports and a pooled report are generated by the pipeline.